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rat col iii elisa kits  (Cusabio)


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    Cusabio rat col iii elisa kits
    Rat Col Iii Elisa Kits, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+col+iii+elisa+kits/Mouse+Collagen+Type+%E2%85%A2%2CCol+%E2%85%A2+ELISA+KIT/10__1093_slash_burnst_slash_tkaf022-88-20-24
    Average 93 stars, based on 4 article reviews
    rat col iii elisa kits - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mechanical stretching enhances the cellular and paracrine effects of bone marrow mesenchymal stem cells on diabetic wound healing
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) was conducted to quantify prohealing ingredients, such as growth factors and collagens, secreted by BMSCs and BMSC cell sheets. .. Specifically, rat VEGF ELISA kits (Abcam, ab100751), rat TGF-β ELISA kits (Abcam, ab119557), rat Col-I ELISA kits (Abcam, ab210579), and rat COL-III ELISA kits (Cusabio, CSB-E07925m) were used to quantify the VEGF, TGF-β, Col-I, and COL-III concentrations, respectively, according to the product instructions. .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, blocked with 10% goat serum albumin, and stained with Ki67, CD105, MYH9, ZO-1, and filamentous actin (F-actin, 1:200; Abcam, Cambridge, UK).

    Article Title: Mechanical stretching enhances the cellular and paracrine effects of bone marrow mesenchymal stem cells on diabetic wound healing
    Article Snippet: D ow nloaded from https://academ ic.oup.com /burnstraum a/advance-article/doi/10.1093/burnst/tkaf022/8052958 by guest on 12 M arch 2025 UN CO RR EC TE D MA NU SC RI PT Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (ELISA) was conducted to quantify prohealing ingredients, such as growth factors and collagens, secreted by BMSCs and BMSC cell sheets. .. Specifically, rat VEGF ELISA kits (Abcam, ab100751), rat TGF-β ELISA kits (Abcam, ab119557), rat Col-I ELISA kits (Abcam, ab210579), and rat COL-III ELISA kits (Cusabio, CSB-E07925m) were used to quantify the VEGF, TGF-β, Col-I, and COL-III concentrations, respectively, according to the product instructions. .. Immunofluorescence of BMSCs The cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, blocked with 10% goat serum albumin, and stained with Ki67, CD105, MYH9, ZO-1, and filamentous actin (F-actin, 1:200; Abcam, Cambridge, UK).



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    rat col iii elisa kits  (Cusabio)
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    Cusabio rat col iii elisa kits
    Rat Col Iii Elisa Kits, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+col+iii+elisa+kits/Mouse+Collagen+Type+%E2%85%A2%2CCol+%E2%85%A2+ELISA+KIT/10__1093_slash_burnst_slash_tkaf022-88-20-24
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    col iii  (Novus Biologicals)
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    rat collagen type iii elisa kit  (Cusabio)
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    Col <t>I/III</t> production from co-cultures with and without mechanically loaded myoblasts. ( A , D ) Relative mRNA expression of Col 1a1 and Col 3a1 in tenocytes. ( B , E ) Quantification of Col 1 content in tenocyte lysate by <t>ELISA.</t> ( C , F ) Quantification of Col I/III content and the ratio in the cell culture medium. ( G ) Immunofluorescence staining of Col I on tenocytes. Teno ctrl: tenocytes alone, Co ctrl: tenocytes co-cultured with myoblasts, Dyn: tenocyte co-cultured with dynamically loaded myoblasts, Stat: tenocyte co-cultured with statically loaded myoblasts. Data are represented as mean ± standard deviation. n = 3. Student’s t -test was performed when comparing Teno ctrl and co ctrl. One-way ANOVA with Tukey’s multiple comparisons was performed when comparing between Co ctrl, Dyn and Stat groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    collagen type iii level  (Cusabio)
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    Cusabio collagen type iii level
    In the in vitro model of arachnoid cell fibrosis, the expression of miR-30a decreased, and the expression of TRAF3IP2 was upregulated. (a) Differential mRNA expression of miR-30a, TRAF3IP2, and TGF-β1 in arachnoid cells between the fibrotic group and control group (** P < 0.01 compared with the control group). (b) Differential protein expression of TRAF3IP2, TGF-β1, and α-SMA in arachnoid cells between the fibrotic and control groups (** P < 0.01 compared with the control group; * P < 0.05 compared with the control group). (c) Differential content of collagen I and <t>collagen</t> <t>III</t> in the model of arachnoid cell fibrosis (** P < 0.01 compared with the control group).
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    Image Search Results


    Col I/III production from co-cultures with and without mechanically loaded myoblasts. ( A , D ) Relative mRNA expression of Col 1a1 and Col 3a1 in tenocytes. ( B , E ) Quantification of Col 1 content in tenocyte lysate by ELISA. ( C , F ) Quantification of Col I/III content and the ratio in the cell culture medium. ( G ) Immunofluorescence staining of Col I on tenocytes. Teno ctrl: tenocytes alone, Co ctrl: tenocytes co-cultured with myoblasts, Dyn: tenocyte co-cultured with dynamically loaded myoblasts, Stat: tenocyte co-cultured with statically loaded myoblasts. Data are represented as mean ± standard deviation. n = 3. Student’s t -test was performed when comparing Teno ctrl and co ctrl. One-way ANOVA with Tukey’s multiple comparisons was performed when comparing between Co ctrl, Dyn and Stat groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production

    doi: 10.3390/ijms222313089

    Figure Lengend Snippet: Col I/III production from co-cultures with and without mechanically loaded myoblasts. ( A , D ) Relative mRNA expression of Col 1a1 and Col 3a1 in tenocytes. ( B , E ) Quantification of Col 1 content in tenocyte lysate by ELISA. ( C , F ) Quantification of Col I/III content and the ratio in the cell culture medium. ( G ) Immunofluorescence staining of Col I on tenocytes. Teno ctrl: tenocytes alone, Co ctrl: tenocytes co-cultured with myoblasts, Dyn: tenocyte co-cultured with dynamically loaded myoblasts, Stat: tenocyte co-cultured with statically loaded myoblasts. Data are represented as mean ± standard deviation. n = 3. Student’s t -test was performed when comparing Teno ctrl and co ctrl. One-way ANOVA with Tukey’s multiple comparisons was performed when comparing between Co ctrl, Dyn and Stat groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Collagen I and collagen III were assessed using Rat Collagen Type I ELISA kit (Cusabio, Wuhan, China, #CSB-E08084r) and Rat Collagen Type III ELISA kit (Cusabio, Wuhan, China, #CSB-E07924r) according to the manufacturer’s protocol.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Immunofluorescence, Staining, Standard Deviation

    In the in vitro model of arachnoid cell fibrosis, the expression of miR-30a decreased, and the expression of TRAF3IP2 was upregulated. (a) Differential mRNA expression of miR-30a, TRAF3IP2, and TGF-β1 in arachnoid cells between the fibrotic group and control group (** P < 0.01 compared with the control group). (b) Differential protein expression of TRAF3IP2, TGF-β1, and α-SMA in arachnoid cells between the fibrotic and control groups (** P < 0.01 compared with the control group; * P < 0.05 compared with the control group). (c) Differential content of collagen I and collagen III in the model of arachnoid cell fibrosis (** P < 0.01 compared with the control group).

    Journal: Translational Neuroscience

    Article Title: Decreased MiR-30a promotes TGF-β1-mediated arachnoid fibrosis in post-hemorrhagic hydrocephalus

    doi: 10.1515/tnsci-2020-0010

    Figure Lengend Snippet: In the in vitro model of arachnoid cell fibrosis, the expression of miR-30a decreased, and the expression of TRAF3IP2 was upregulated. (a) Differential mRNA expression of miR-30a, TRAF3IP2, and TGF-β1 in arachnoid cells between the fibrotic group and control group (** P < 0.01 compared with the control group). (b) Differential protein expression of TRAF3IP2, TGF-β1, and α-SMA in arachnoid cells between the fibrotic and control groups (** P < 0.01 compared with the control group; * P < 0.05 compared with the control group). (c) Differential content of collagen I and collagen III in the model of arachnoid cell fibrosis (** P < 0.01 compared with the control group).

    Article Snippet: ELISA kit was used to detect the TGF-β1 level (EK0514, Wuhan Boster Biological Technology, Ltd, China), collagen type I level (CSB-E08084r, CUSABIO, China), and collagen type III level (CSB-E07924r, CUSABIO, China) following the protocols constructions.

    Techniques: In Vitro, Expressing, Control

    MiR-30a inhibits subarachnoid fibrosis through inhibiting TRAF3IP2. (a and b) The mRNA and protein expression of miR-30a, TRAF3IP2, TGF-β1 and α-SMA from different groups (** P < 0.01 compared with the control group; *** P < 0.001 compared with the control group). (c) The content of collagen I and collagen III from different groups (*** P < 0.001 compared with the control group. ** P < 0.01 compared with the control group; * P < 0.05 compared with the control group).

    Journal: Translational Neuroscience

    Article Title: Decreased MiR-30a promotes TGF-β1-mediated arachnoid fibrosis in post-hemorrhagic hydrocephalus

    doi: 10.1515/tnsci-2020-0010

    Figure Lengend Snippet: MiR-30a inhibits subarachnoid fibrosis through inhibiting TRAF3IP2. (a and b) The mRNA and protein expression of miR-30a, TRAF3IP2, TGF-β1 and α-SMA from different groups (** P < 0.01 compared with the control group; *** P < 0.001 compared with the control group). (c) The content of collagen I and collagen III from different groups (*** P < 0.001 compared with the control group. ** P < 0.01 compared with the control group; * P < 0.05 compared with the control group).

    Article Snippet: ELISA kit was used to detect the TGF-β1 level (EK0514, Wuhan Boster Biological Technology, Ltd, China), collagen type I level (CSB-E08084r, CUSABIO, China), and collagen type III level (CSB-E07924r, CUSABIO, China) following the protocols constructions.

    Techniques: Expressing, Control